Newport Green, a fluorescent sensor of weakly bound cellular Zn2+: competition with proteome for Zn2+
نویسندگان
چکیده
منابع مشابه
Newport Green, a fluorescent sensor of weakly bound cellular Zn(2+): competition with proteome for Zn(2).
Newport Green (NPG) is a recognized sensor of cellular Zn(2+) that displays fluorescence enhancement upon binding to Zn(2+). Because of its modest affinity for Zn(2+), the extent of its capacity to bind cellular Zn(2+) is unclear. The present study investigated the range of reactivity of NPG(ESTER) with cells, isolated (Zn)-proteome, and model Zn-proteins. The sensor accumulated in pig kidney L...
متن کاملA ratiometric fluorescent sensor for phosphates: Zn2+-enhanced ICT and ligand competition.
A pyrene-terpyridine-Zn conjugate has been synthesized and characterized, where Zn2+ acts as an electron acceptor to enhance molecular ICT with a large emission red-shift (>100 nm). It showed a ratiometric fluorescence change upon addition of phosphate anions in buffered aqueous solution. The selective response to phosphates or pyrophosphates involved ICT and ligand competition processes.
متن کاملMonitoring apoptosis with fluorescent Zn2+-indicators.
Apoptosis, a mechanism of programmed cell death that removes superfluous and harmful cells, is important both during development and in tissue homeostasis. Although Zn2+ is believed to be critical in apoptosis, the precise details of its role have yet to be elucidated. The macrocyclic Zn2+ ligand dansylamidoethylcyclen [L1*(HCl)4*(H2O)2], which is found primarily in a diprotonated form (H2L1), ...
متن کاملDetection of Zn2+ release in nitric oxide treated cells and proteome: dependence on fluorescent sensor and proteomic sulfhydryl groups.
Nitric oxide (NO) is both an important regulatory molecule in biological systems and a toxic xenobiotic. Its oxidation products react with sulfhydryl groups and either nitrosylate or oxidize them. The aerobic reaction of NO supplied by diethylamine NONOate (DEA-NO) with pig kidney LLC-PK1 cells and Zn-proteins within the isolated proteome was examined with three fluorescent zinc sensors, zinqui...
متن کاملReaction-based fluorescent sensor for investigating mobile Zn2+ in mitochondria of healthy versus cancerous prostate cells.
Chelatable, mobile forms of divalent zinc, Zn(II), play essential signaling roles in mammalian biology. A complex network of zinc import and transport proteins has evolved to control zinc concentration and distribution on a subcellular level. Understanding the action of mobile zinc requires tools that can detect changes in Zn(II) concentrations at discrete cellular locales. We present here a zi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Metallomics
سال: 2016
ISSN: 1756-5901,1756-591X
DOI: 10.1039/c5mt00167f